Huashi Baidu formula alleviates lipopolysaccharide-induced inflammation and acute lung injury in mice by targeting nuclear factor κB/phosphatidylinositol 3-kinase and peroxiredoxin 5

Huashi Baidu formula alleviates lipopolysaccharide-induced inflammation and acute lung injury in mice by targeting nuclear factor κB/phosphatidylinositol 3-kinase and peroxiredoxin 5

Blog Article

Abstract.Background.Acute respiratory distress syndrome induced by acute lung injury (ALI) is the main cause for the high mortality of coronavirus disease 2019 (COVID-19).Huashi Baidu formula (HSBD) with the effects of eliminating dampness, clearing heat, ventilating lung, and removing toxin has been proven to be effective in the treatment of COVID-19, especially in severe cases.

However, the underlying mechanism and target proteins of HSBD remain unclear.Objective.To provide evidence and decipher the mechanism of HSBD in alleviating inflammation and ALI.Materials and Methods.

A mouse model of ALI was induced by lipopolysaccharide (LPS), and hematoxylin-eosin staining was used to examine the protective effects of HSBD on the model mice.The cellular thermal shift assay and Espresso Machine (Single-Serve) proteomics analysis were used to predict the target proteins.Furthermore, the A549 cells with peroxiredoxin 5 (PRDX5) knockdown were established to validate the predicted proteins.Results.

Huashi Baidu formula treatment mitigated ALI and inflammatory cytokine dysfunction in LPS-induced mice, thus exerting a therapeutic effect on COVID-19.Huashi Baidu formula could serve as a therapeutic agent to alleviate inflammation and lung injury via nuclear factor κB and phosphatidylinositol 3-kinase signaling and interleukin 17 inhibition as well as R/C Helicopter targeting PRDX5, which could be one of the promising targets for treating inflammation.In the A549 cell line with PRDX5 knockdown (si-PRDX5), the anti-inflammation effects of HSBD, including reversing LPS-induced increase in the nitric oxide level and reduction in the hydrogen peroxide content, were attenuated.Thus, HSBD protected A549 cells from LPS-induced inflammation mainly by targeting PRDX5.

Conclusions.Huashi Baidu formula alleviates ALI by targeting nuclear factor κB/phosphatidylinositol 3-kinase and PRDX5, as well as inhibiting the immune response induced by IL-17.